DWARF 53 acts as a repressor of strigolactone
signalling in rice (Nature 504: 401-405, 2013)
LEUNIG_HOMOLOG Mediates MYC2-Dependent Transcriptional Activation in Cooperation with the Coactivators HAC1 and MED25
Yanrong You, Qingzhe Zhai, Chunpeng An, Chuanyou Li

The Plant Cell

Groucho (Gro)/Tup1 (Thymidine uptake 1) family proteins are evolutionarily conserved transcriptional coregulators in eukaryotic cells. Despite their prominent function in transcriptional repression, little is known about their role in transcriptional activation and the underlying mechanism. Here, we report that the plant Gro/Tup1 family protein LEUNIG_HOMOLOG (LUH) activates MYC2-directed transcription of JAZ2 and LOX2 via the Mediator complex coactivator and the histone acetyltransferase HAC1. We show that the Mediator subunit MED25 physically recruits LUH to MYC2 target promoters, which then links MYC2 with HAC1-dependent histone H3K9 acetylation to activate JAZ2 and LOX2. Moreover, LUH promotes hormone-dependent enhancement of protein interactions between MYC2 and its coactivators MED25 and HAC1. Our results demonstrate that LUH interacts with MED25 and HAC1 through its distinct domains, thus imposing a selective advantage by acting as a scaffold on MYC2 activation. Therefore, the function of LUH in regulating jasmonate signaling is distinct from the function of TOPLESS, another member of the Gro/Tup1 family that represses MYC2-dependent gene expression in the resting stage.

©2008-2009 中科院遗传发育所植物基因组学国家重点实验室 版权所有 京ICP备09063187号
邮编: 100101 邮件:plantgenomics@genetics.ac.cn , 电话:010-64806595