DWARF 53 acts as a repressor of strigolactone
signalling in rice (Nature 504: 401-405, 2013)
MED25 Connects Enhancer-Promoter Looping and MYC2-Dependent Activation of Jasmonate Signaling
Hang Wang, Shuyu Li, Yan’an Li, Yiran Xu, Yunhao Wang, Ruoxi Zhang, Wenjing Sun, Qian Chen, Xiu-jie Wang, Chuanyou Li, Jiuhai Zhao

Nature Plants


The lipid-derived hormone jasmonate (JA) regulates plant immunity and adaptive growth by triggering a genome-wide transcriptional programme. In Arabidopsis thaliana, JA-triggered transcriptional programming is largely orchestrated by the master transcription factor MYC2. The function of MYC2 is dependent on its physical interaction with the MED25 subunit of the Mediator transcriptional co-activator complex. Here we report the identification of JA enhancers (JAEs) through profiling the occupancy pattern of MYC2 and MED25. JA regulates the dynamic chromatin looping between JAEs and their promoters in a MED25-dependent manner, while MYC2 auto-regulates itself through JAEs. Interestingly, the JAE of the MYC2 locus (named ME2) positively regulates MYC2 expression during short-term JA responses but negatively regulates it during constant JA responses. We demonstrate that new gene editing tools open up new avenues to elucidate the in vivo function of enhancers.

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